Yes, the IC₅₀ value of a drug can indeed vary across different experiments, even when using the same cell line.
- 1.5- to 3-fold variability is often considered normal in biological assays.
- >3-fold differences may suggest technical issues or biological outliers and warrant further investigation.
Hi Akhilesh!
Yes, the IC₅₀ value of a drug can vary across different experiments using the same cell line due to multiple factors, including experimental conditions, passage number, seeding density, assay duration, serum concentration, and drug stability. Variability in reagent quality, incubation time, and differences in instrument sensitivity can also contribute to fluctuations. Generally, IC₅₀ values can differ by up to 2-5 fold, though in some cases, variations beyond this range may occur due to uncontrolled experimental parameters. To ensure reproducibility, maintaining consistent assay conditions, cell culture protocols, and internal controls is essential.
May be we can discuss in depth as per your need..
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