I want to isolate DNA, RNA, and protein from rice leaves. Trizol does all three, which makes it a good contender for me. However, I want to run the protein samples through MRM-MS and heard a colleague mention that Trizol might not be compatible with the MS.
Does anyone know whether this is true or not? If so, any ideas on how to proceed?
Thanks :)
Hallo, am not very familiar with DNA/RNA extraction. But what maybe considered incompatible with MS are:
i) Non volatile compounds
ii) Ion suppression compounds,
From the extraction process, several rinsing steps with 75% ethanol are involved, only minimal traces of of the Trizol maybe left if any.
Hi,
You can very well use.
You may need to go for buffer exchange before doing your digestion. you can use cut off membrane based system for the buffer exchange; you can transfer your extracted proteome into Ammonium bicarbonate based buffer.
you can refer this paper
http://www.sciencedirect.com/science/article/pii/S1874391915301974
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