Joel Christie

5 Questions 11 Answers 0 Followers

Questions related from Joel Christie

How to use MitoTracker Green FM for HEK293 cells?

Hi, I am planning to use the MitoTracker Green FM dye for HEK293 cells, have you used HEK293 for such experiment? What concentration normally one uses for HEK293 cells and what should be the...

12 October 2017 2,341 5 View

Can I use Ammonium Bicarbonate as buffer if I dont have TEAB for iTRAQ experiment?

I came to know that Tris having primary amine group may interact with iTRAQ labels, and TEAB is an advised buffer to be used post precipitation; but if I don't have TEAB at present can I use...

25 November 2016 3,554 3 View

Does anyone have any idea about the reverse protein hits generated by Protein Pilot?

I am facing one problem with identification carried out using Protein Pilot, I get many hits whose description shows reverse protein. Does anyone have any explanation for this? Use of Mascot...

18 September 2016 203 6 View

How to identify retention time of any particular peptide in any given LC MS run?

I want to quantify certain proteins using label free peptide peak intensity based method; for this I feel first of all I should know the retention time of identified peptides, and after...

01 May 2016 9,734 4 View

What does zero peptide mean in protein pilot data analysis?

In Proteomics, while doing analysis on protein pilot after running my sample on mass spec; I got plenty of protein IDs which are having zero peptide identification to the library. I don't...

15 January 2016 5,066 3 View