I quantifying the Dopa mine,Serotonin,Norephinephrin by HPLC with Electrochemical detection (ECD). I want quantify Epinephrine Acetylcholin,GABA,Glutamate,endorphin and Histamines.
Jacques Callebert
I agree with John. You can use the same protocol with HPLC-ECD for quantification of epinephrine. But for quantification of gaba and glutamate, you have to use a method of OPA derivatization, for acetylcholine you need to apply a post column IMER.
All these methods use different columns.
And for endorphin or histamin, the problem is the same : these compounds are electroactive (they can be measured by ECD) but in completely different conditions from those used for catechols.
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