Please let me know can I ues RPMI to BEAS-2B cells for culturing and which is the best freezing medium for this cells?
Hello
Yes you can use RPMI, HAM, DMEM mediums with BEAS-2B
discussions in RG links will helpful
https://www.researchgate.net/post/Can_anyone_help_with_a_BEAS-2B_cell_line/1
Hi, thank you so much
Hi, you can you RPMI for BEAS-2B but it will be better if u adapt the cell line with old and new media in a 75:25 ratio and 50:50 ratio and so on. Use the following link for better understanding.
http://www.baculovirus.com/protocols/media_adaptation.htm
NO! Absolutely not. Media containing FBS induces EMT in BEAS-2B. You need to use a serum free specialty media such as LHC-9.
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