Kushal Kandhari

3 Questions 9 Answers 0 Followers

Questions related from Kushal Kandhari

Is it better to store protein lysates in -20°C or should i store it in -80°C to minimize the degradation?

I do western blotting with these lysates and i just want to minimize the degradation of protein as much as possible and i also want to know whether the storing temperature (-20 or -80) have any...

11 November 2016 8,741 13 View

Why are my MCF-7 breast cancer cells not growing properly?

I am working on MCF-7 cells...whenever i revive the cells they get attached and for the first two days their morphology is very good..(edges glow when u view under microscope and cells seem great...

06 June 2015 4,970 12 View

Troubleshooting western blot for 200 kD Protein. Can anyone help?

I am working in the area of EMT in Cancer cells. I have to perform western blot of an important marker of EMT i.e. Zeb1. The problem is i have tried several times but i am unable to get western...

01 January 1970 2,457 9 View