Hi everybody,
I want to co transform 2 genes that are in different plasmids into one Cell, they are both in a same plasmid, so if I do they are incompatible (they have pBR322 ori)
So I need to use another plasmid for one of the genes (pA15 ori), to don't waste time in ordering pCyC plasmid, I want to use pLySs (that have pA15 ori).
However, I read that this plasmid in not for cloning but for expression of T7 RNA terminator.
Can I still use it to clone my gene?
Thanks for your help.