I am using the TeSR-E8 medium for the maintenance and growth of iPSCs. I made the aliquotes of complete medium without adding Pen/Strp and Normocine, and stored at -20C. On need, I thaw one of them and store the remaining volume at 4C where it remains free of contamination for one week. But it gets contaminated after that even though I use and store the vial very carefully. Can I please get any suggestions regarding the use and store of stem cell medium? Is it ok for iPSCs to use medium containing Pen/Str and Normocine?
Thank You Seema! Do you have idea about antifungal reagent like Normocine?
I never culture stem cells with antibiotics. If you cannot keep your cultures clean, then you need to correct the reason why you continue to contaminate them rather than trying to mask the contamination.
If you feel that you need antibiotics and antimycotics to prevent continued contaminations, then the odds are good that your cultures are, or will become, contaminated with mycoplasma as well.
Hello
We routinely culture iPSC in medium without any antibiotics but If you need to use them, it is recommended to use lower concentrations ( 1% pen/strep )
Good Luck
right th antibiotic concentration should be low. it is around 1%
pen/strep
Thank you all for your valuable suggestions! I will try to be careful to avoid contamination.
All Pen/Strep solutions I ever have bought were 100x solutions, so it would end up being 1% in the media.
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