I am working on developing 3D cell cultures of tumor cells and I noticed that there are different methods, like liquid overlay, air interface culture, hanging drop technique, and scaffold-mediated approaches.
As for the liquid overlay approach, the general idea is to create a surface that cells cannot adhere onto. I am curious about whether I can use matrigel to replace agar/agarose or HEMA for the development of 3D cell culture?
Hi Weizhong,
One alternative is plant nanocellulose hydrogels (GrowDex) which are well-defined and animal-free matrices for 3D cell culture. Cells can grow in all three dimensions in the hydrogel where the cellulose nanofibers form the network and provide physical support for the cells. This is one step closer to in vivo situation, compared to suspension cultures on ultra low attachment wells where is no fiber network. Please check a couple of publications with cancer cell data:
Article MUG-Mel2, a novel highly pigmented and well characterized NR...
Article β1 Integrin is Essential for Fascin‐Mediated Breast Cancer S...
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