Can I use food grade BHT standard for my TBARS assay?

07 March 2022 1 7K Report

I will be conducting the TBARS asay and it says to use BHT as the positive control. However, upon checking primary and secondary reference standards of BHT is too expensive. Can I use a food grade standard instead?

Shail K Chaube

I would not suggest you to use the food grade BHT standard for your TBARS assay since the purity of BHT as a positive control is one of the concerns. If you are not in position to afford BHT (high purity grade; analytical standard), you can follow the protocol published in Methods in Enzymology, Volume 52, 1978, Pages 302-310. The link is given below:

Microsomal lipid peroxidation. Article Microsomal Lipid Peroxidation

Good luck

Badges
Science topic

More Duri Eun's questions See All

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,140 3 View

DAB fail-regions in DAB positive area. Why?

I am recently observing DAB staining fail-regions (red circles or red arrows on pictures) on my DAB stained slides. Does anyone have ideas why I am getting this? I can see only hematoxylin stained...

16 May 2024 693 0 View

What to do when the autosampler needle was lowered, it hit a foreign object causing excessive force to be exerted to it?

I suddenly got an error on our HPLC system saying : when the autosampler needle was lowered, it hit a foreign object causing excessive force to be exerted to it. What could have possibly...

27 July 2023 4,325 0 View

Coach Training Learning Coaching ?

Coach Training Learning Coaching

16 May 2023 5,174 0 View

How to compute FRAP value?

I am confused on how to present my data on the Ferric reducing antioxidant power FRAP assay. I have prepared my samples on a concentration range but i do not know how to present in percentage to...

31 October 2022 7,335 4 View

How do you get an extraction percent yield using dry weight?

May I know how to compute for plant extraction yield with regards to its dry weight? For example, I used fresh samples with 80% Moisture content. 150g leaves were macerated and the total extract...

17 October 2022 1,127 5 View

Can I quantify a certain compound using HPLC if I keep getting merged & splitting peaks?

I have a problem with my HPLC analyses. I keep getting split peaks and I am not sure if I can quantify those compounds. I do have reference standards and I should do a calibration curve to be able...

12 October 2022 9,266 4 View

What are the appropriate DPPH and FRAP assay values?

Why is DPPH expressed as IC50 and not EC50? Or could they be used interchangeably? Also for FRAP assay I am confused as some studies used EC50 and some used Ferrous Sulfate equivalents. Which...

17 September 2022 265 1 View

Should TFC always be lower than TPC?

I am getting the total phenolics content and total flavonoids content of a sample. Should the TFC be always lower in value? I know that flavonoids are prt of phenolics but since the protocols and...

20 August 2022 147 3 View

How to quantify compounds in LC MS?

Im curious as to whether the software for LCMS (Labsolutions from Shimadzu) already has a quantification feature or do I have to compute and compare the AUCs to the standards manually? If so, what...

04 July 2022 2,025 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,799 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,596 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,829 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

What factors could be contributing on low tumor take in female Nude mice?

Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

05 July 2024 5,859 1 View