Hello!
I need to made some buffer for phenol-chloroform extractions (Saline EDTA) and TE buffer. However I only found in the lab some Ethylenediaminetetraacetic acid (MW: 292) instead of disodium EDTA (MV: 372).
Can someone tell me if I can use the first one instead of the second? And if not, if there is a way to make disodium EDTA starting from EDTA?
Thanks in advance!
Hello Vincent,
Disodium EDTA has a molecular weight of 336.2 g/mol.
(be sure what you have , either your molecular weight is wrong hence it's disodium, or your molecular weight is right hence it's dipotassium i guess)
The differences are the solubility and the pH of the resulting solution. Although If you will add NaOH to your buffer, the chemical composition of the resulting buffer will be the same in case of using both.
Kind regards.
Hello Vincent,
Disodium EDTA has a molecular weight of 336.2 g/mol.
(be sure what you have , either your molecular weight is wrong hence it's disodium, or your molecular weight is right hence it's dipotassium i guess)
The differences are the solubility and the pH of the resulting solution. Although If you will add NaOH to your buffer, the chemical composition of the resulting buffer will be the same in case of using both.
Kind regards.
The right solution is using disodium edta right rather than trying to make it with NaOH and EDTA . To understand you may follow the practice in http://www.protocol-online.org/biology-forums/posts/26749.html. pH and times to get a solution would blurry any attempt of do your self.
I'm guessing instead of disodium EDTA he ment dissodium EDTA dihydrate, which has a molecular weight of 372.24.
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