Hi I am currently doing a quantitative real time PCR on my samples. I am quantifying the viral load (WSSV) on different crustaceans using the product KAPA SYBR FAST qPCR. I just want to ask if anyone here experience using the same product for quantifying DNA? May I know if you are adding dUTPs on your samples. This is because I've tried adding dUTPs on my master mix and the melting curve went well. However when I tried to use the SYBR FAST mastermix only (w/o dUTPs) I'm getting two peaks on my Melting curve. I know that getting two peaks on melting curve maybe due to the primers, but how come when I added dUTPs on my mix the melting curve went well?