Can I us DMEM / F12 50/50 with L-Glutamine +10%FBS when growing HepG2 cells. Currently, they are growing in standard EMEM (ATCC). also low or high sugar, I am getting positives for both?
Also, Has anyone ever used IMDM on these cells?
Hello Antonio,
HepG2 cells can be cultivated in different media, for example:
1) DMEM + 10% FBS + 100 µg mL−1 penicillin and 10 µg mL−1 streptomycin (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375833/); (https://www.encodeproject.org/documents/01ccc7af-f2f3-4d70-bc17-cd82757ef0ca/@@download/attachment/HepG2_protocol.pdf);
2) -high-glucose Dulbecco's modified Eagle's medium, containing 25mM glucose and 1mM sodium pyruvate and supplemented with 5mM N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), 10% FBS, and penicillin-streptomycin (pen-strep; 500 μg/ml final concentration);
-DMEM deprived of glucose supplemented with 5.5mM glucose, 5mM HEPES, 10% FBS, 1mM sodium pyruvate, and pen-strep (https://academic.oup.com/toxsci/article/97/2/539/1650344/Circumventing-the-Crabtree-Effect-Replacing-Media);
3) DMEM medium supplemented with 10% FBS, 2 mM L-glutamine and 100 units per ml penicillin/streptomycin (https://www.nature.com/articles/ncomms11776);
4) serum free medium DMEM/F12 (50/50) supplemented with 1 × N2 ((Gibco Invitrogen)), 20 mM L-Glutamine, 0.5 U/ml penicillin, and 0.5 U/ml streptomycin (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC272931/#B21);
5) DMEM/Ham’s F-12 (50:50) (DMEM/F12 supplemented with 10% FBS, l-glutamine, and 15 mm HEPES (https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/endo/148/11/10.1210_en.2007-0376/2/endo5295.pdf?Expires=1497911397&Signature=DOA5FiuZnFAnfCXJ0z~vsETieT5CcPLOOa9YLWXNP20qIRqNnvVNnwOn~uCremThGwNWO40~CUTsMsHt0wOplV7fNHqAfOG7kl~9e6o4QoIBtKSiNCL4ihGiGgA2qMywtq3LUoezaIiGD6PclGWw4X-AiaTFHe9YsbF2t5NwVRmk0naafWJrgkbkPRXv50R4Bdrj7vSKbaj2IWA2eeWtBBTHMVaYogD6YpwCpUgoogTPvjQn0ij5Q~FCaJCZMzoTC-ivYqtzy8QgYyDmrX7FVYmgvsYfzwXg8dgDQi8nue3h2M0PsL7qRlCLPG0pBIxbufvWr~LudpLzAqRiCFME6g__&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q);
6) DMEM/F12 , 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin (https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-89);
7) IMDM (MediaTech) and supplemented with 10% fetal bovine serum (Mediatech) (http://csmedia2.corning.com/LifeSciences/Media/pdf/poster_2009_ala_caliper_ultraweb.pd).
8) MEM supplemented with 10% FBS, 200 U mL#1 penicillin, 200 mg mL#1 streptomycin, 1 mM sodium pyruvate, and 1 mM nonessential aminoacids, then this medium was changed on IMDM supplemented with 20% FBS, 200 U mL#1 penicillin, 200 mg mL#1 streptomycin, 1 mM nonessential amino acids, 0.5 U mL#1 insulin, 14 ng mL#1 glucagon, and 100 nM dexamethasone (http://www.bme.ucdavis.edu/revzin/files/2010/11/2009-Directing-hepatic-differentiation-of-embryonic-stem-cells-.pdf).
Best wishes,
Elena
Hello
For HepG2 culture use one of these method :
# DMEM/F12 (50/50) supplemented with 1 × N2, 20 mM L-Glutamine, 0.5 U/ml penicillin, and 0.5 U/ml streptomycin.
# DMEM-F12 (50:50) supplemented with 10% (v/v) heat-inactivated fetal calf serum, penicillin G (100 U/ml), and streptomycin (100 μg/ml).
# DMEM with glutamine, pyruvate and 10% FBS..
OR culture them in IMDM supplemented with 10% FBS and incubat at 37º and 5% CO2.
Ref ( https://www.ncbi.nlm.nih.gov/pmc/articles/PMC272931/#B21 )
Ref ( http://www.jlr.org/content/42/9/1402.full.html )
http://www.hepg2.com/
Dulbecco’s Modification of Eagle’s Medium (DMEM)/F12 with 2 mM L-Glutamine and 15 mM HEPES buffer growth medium supplemented with 10% fetal bovine serum (FBS) and and 1penicillin/streptomycin (pen-strep; 500 μg/ml final concentration)
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