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Questions related from Antonio Bifero
Making mM Ethanol concentrations? Hi All! Looking for best practices in making stock mM EtOH and Paracetemol concentrations. Here is what I need for both5, 10, 20, 30 and 40mM drug stock...
14 December 2017 9,498 0 View
Can someone help me out with the details. I am trying to create a routine calculation for hemocytometer and volume dilutions for my experiments. Here is what I have. Culture HepG2 in EMEM=10%FCS...
07 December 2017 6,808 6 View
I am trying to put together dose response curves for both EtOH and Paracetamol using HepG2 cells and am unsure if they express CYP2E1. Some background seems to say they do not (MV5? cells). If...
28 September 2017 3,635 2 View
Can I us DMEM / F12 50/50 with L-Glutamine +10%FBS when growing HepG2 cells. Currently, they are growing in standard EMEM (ATCC). also low or high sugar, I am getting positives for both? Also,...
17 June 2017 2,455 4 View
