I am interested in synthesizing a certain short, linear, cationic peptide via solid phase peptide synthesis. Is it best to do toxicity assay in vivo using C. elegans for a basic experiment testing? I can't find a journal doing peptide toxicity testing using this nematode.
Need some insights. Thank you!
It is best when the factors considered are time, cost, and metabolic function. You have a peptide but you wish to know if it is likely to remain effective when taken orally (where peptidases would be encountered) in the fastest way possible, or if toxicity is based on lifespan and vertebrates simply aren't time-friendly in this context, and meeting deadlines is a must. It is not best when the factor considered is external validity, as higher in vivo models more closely approximate human experience, and other modes of administration (intravenous, intraperitoneal, subcutaneous) are made possible. It is more often a good start. Here is an article showing how a cocoa peptide was evaluated in transgenic C. elegans: Article A Cocoa Peptide Protects Caenorhabditis elegans from Oxidati...
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Yes you can. There are people who tested novel drugs / proteins / peptides using C. elegans as an In Vivo host model as the model is relatively cheaper, easier to do, faster and does not require ethical requirement.
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