In an attempt to determine the effect of an extract on the above two conditions can I use alpha-glucosidases and alpha-amylases assay for both anti diabetes and anti hyperglycemia?
Diabetes is long-term status disease which surpasses hyperglycemia. Hyperglycemia may be a spike in blood glucose levels in response to meal, or to deliberate induction if in experimental animals. So to confirm diabetes in such situation, it must be as a result of sustained high blood glucose levels; if in experimental animals, the hyperglycemia should be sustained over minimum of 48 hours if not 72 hours. Recovery from hyperglycemia may be possible in experimental animals if diabetes is not precipitated, which may be due to wrong choice of inductive chemical, improper preparation and i.p administration of inductive chemical, lower than effective dose of inductive chemical.
Where do you intend to assay these two enzymes?
Assuming that your extract specifically reduces activities in saliva and intestine, leaving intracellular activity completely intact, which will have some benefit as far as diabetes/hyperglycemia is concerned but it is strictly related to ingestion of carbohydrates in meals.
Gluconeogenesis in the liver is a very significant aspect of sustained hyperglycemia in poorly controlled diabetes.
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