Can I use 10 ul of final reaction mix of PCR Instead of 20 ul final reaction?

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Weak DAPI staining after immunohistochemistry - how to improve?

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How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

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Is there any machine to do real time pcr?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

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Javier Gordon Ogembo

Yes you can

Zhou Jiang

You mean Real-time PCR？ Yes you can,the volume decrease is equal to all the genes

Mangesh Moharil

Yes you can by minimizing the quantities of all component in appropriate proportions, we use it for SSR markers

Pratima Chawla

It depends, if you are doing PCR only for analysis then u can use 10ul. just u have to take care of final concentration of components of master mix.

Lorraine Michelet

Yes you can. You just have to adujst the volume of each component to get the good concentration.

Oadi Matny

I will try it today and see the result, Thanks for All

Thanks for All, it work with good result,

Amanda O'Donnell

As you can see, it is possible. We do it to save money in our lab too.

Can I just add a word of warning though- your pipetting errors will be more of a problem the smaller your final volume is. Keep an eye on the tightness of your replicates otherwise reducing the volume may be a false economy.