What type of assay are you using to quantify your enzymes?  Do your enzymes need to retain activity or are you using Western blotting (where you do not need native protein)?  For Western blotting, I use 3:1 RIPA buffer:brain tissue mass for homogenization.  If this makes to much foam during homogenization then I use the same ration of 100 mM Tris (7.5), 150 mM NaCl, with 1x protease inhibitor cocktail and 1U/ml DNase for the homogenization step.  Then I dilute the homogenate 1:1 with RIPA buffer.  After a 30 minute incubation on ice, I sonicate 2x 30 seconds on ice with 2 minutes between pulses.  Finally, centrifuge to remove insoluble material.

thanks ....for the answer....

Dear

Yes of course you can especially if you want dose the enzymes activities (oxidative stress ).

Good luck

thanks.. sir...