How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

What is wrong with my western blot transfer system? (smearing is occured)?

I'm now doing western blotting for several proteins. but transfer is not perfectly happened. bands are looks like smeared or smudged. And it seems to the lower MW bands are more affected. what did...

06 December 2020 9,153 5 View

Can I autoclave d-mannose?

Hello everyone, I was wondering whether I should autoclave or filter d-mannose solution. In our laboratory, we autoclave glucose separately when we make YPD media for yeast culture. So, I am...

08 October 2020 7,068 1 View

What is the best format to export from igraph to gephi?

I have been using R, igraph, and gephi to create semantic networks. I generally export gml file from R through igraph, then import the gml file into gephi for further adjustment. However, I am now...

06 September 2020 3,906 4 View

Can we relate DC measured resistance to the resistance fitted by equivalent circuit from AC Nyquist plot?

My fitted resistance results from Nyquist are not equal to the one measured from the DC signal. Wondering if it is fair to compare these values measured in different ways.

12 June 2020 7,679 3 View

Does topology from ATB support FF other than Gromos?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

07 June 2020 8,989 2 View

How can I use a molecule from ATB in Gromacs?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

06 June 2020 2,112 4 View

Is there any difference between male and female mice?

I found that many atopic dermatitis research used female mice rather than male ones. I wonder the reason why they prefer female ones.

05 April 2020 9,088 3 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Why is SV40 not considered an oncogenic virus?

Hello, SV40 is not considered an oncogenic virus in this review:Oncogenic viruses and cancer Why is SV40 not considered an oncogenic virus when it produces the large T antigen, which is...

04 December 2020 8,374 5 View

Why do mammalian cells in culture undergo necrosis?

I am observing a large necrotic population in glioblastoma cells that I am culturing in 10% DMEM medium. In spite of changing all parameters of culturing and making sure of the sterilitu, the...

05 November 2020 9,280 3 View

Do ammonium glutamate considered as necessity in salt sloution for Ecoli-based Cell free protein synthesis platform ?

Hello, I am constructing an E-coli based Cell free protein system (CFPS) reagent, but I can't find ammonium glutamate from the local suppliers or from MP biochemical local disruptors. Can I...

13 August 2020 3,691 2 View

Why are my ARPE-19 cells exhibiting this morphology?

Hello! I am culturing ARPE-19 cells (passage 25) in DMEM + Sodium Pyruvate, Glutamine, 1% FBS and observing this phenotype in my cultures. These have been in culture for about 2 months and are...

12 May 2020 8,499 1 View

Is there literature on pre-steady state "lag" for oxidase enzymes?

Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a...

31 March 2020 3,917 2 View

Glioblastoma GL-261 cell line not growing s.c?

Hi has anyone had experiance of GL-261 not growing subcutaneously? I have inoculated 10e6 cells in C57BL/6J female mice and the tumors became palpable after a week cca but have subsequently...

19 February 2020 7,543 2 View