We are looking at whether a peptide: RNA as a complex can bind to a glycosylated protein. We have established with SPR that the Peptide binds to RNA. Is it possible to study the binding of the peptide:RNA complex to a third binding partner with SPR. The experiment I plan to do is to immobilize the third binding partner i.e. the glycosylated protein on the SPR sensor chip and then sequentially flow and dissociate 1) RNA 2) Peptide and 3) RNA+ Peptide mix. Has any one done these kind of interactions with SPR. I am using the Open SPR machine form Nicoya lide
Foud a very old publication which may guide you.
Dallmann, H. G. and McHenry, C. S.; DnaX complex of Escherichia coli DNA polymerase III holoenzyme. Physical characterization of the DnaX subunits and complexes. J.Biol.Chem. (270): 29563-29569; 1995.
Nikhil Kulkarni , SPR signal reflects mass of analyte. With sufficient mass difference between the RNA and the peptide, you might be able to guess or distinguish which analyte was bound or both of analytes were bound.
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