Nikhil Kulkarni

6 Questions 15 Answers 0 Followers

Questions related from Nikhil Kulkarni

Can I study three partner interactions with Surface Plasmon Resonance?

We are looking at whether a peptide: RNA as a complex can bind to a glycosylated protein. We have established with SPR that the Peptide binds to RNA. Is it possible to study the binding of the...

02 February 2020 5,897 2 View

RNA digestion in cell culture supernatant?

I want to digest all RNA in cell culture supernatent. I am planning to do a RNAse A treatment . Are there other recommendations for inactivating RNA?

03 March 2017 4,472 2 View

What free softwares and/or resources are available in order to learn how to dock a ligand (drug) to a protein?

I want to check whether my drug of interest docks into the active site of a nuclear hormone receptor. For this I need expert assistance me with the analysis.

06 June 2014 7,631 3 View

What method do you use for choosing the correct refrence gene?

I was wondering how to select the right reference gene for my experiment. I first thought of using GeNorm but since this is not a free software anymore do you guys have any suggestions ? Also I...

10 October 2012 317 4 View

Change in Ct values over passages in untreated cells?

I have been doing qRT-PCR for TNF gene and I saw that there was a difference in Ct values in the untreated cells over subsequent three passages. When I look at the reference gene beta tubulin it...

10 October 2012 1,419 7 View

q-RT PCR reference gene variation in passages?

I have been doing q RT PCR for almost a year now. I have seen that there are differences in reference gene ( Beta -actin and 18 s rRNA) Ct values in different passages (normal difference being ct...

07 July 2012 8,822 5 View