My extracted RNAs are biotin labelled. And I have used Biotin-Aniline to label RNAs. I want to store these RNAs at -80 degree Celsius for next day processing with streptavidin conjugation.
Yes, you can but is should be ethanol-precipitated, see https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/277/135/11685597910.pdf?msockid=3c180f335eaa679411351d5e5f5b66f1
Thank you dear sir for your valuable answer. I need further clarification from you. Biotin-aniline radicals have increased reactivity towards RNA. Please see this article. ''Expanding APEX2 Substrates for Proximity-Dependent Labeling of Nucleic Acids and Proteins in Living Cells''.
I have labeled RNAs of the cells with these biotin aniline radicals. Now I need to lyse the cells and extract RNA by using TRIzol, precipitate them with ethanol, and add RNase free water and store at -80 degree celsius.
Will this Biotin conjugation with RNA be stable at -80 degree celsius? Will it be used next day to run the 1% agarose denaturing gel and conjugation with streptavidin in Northern Blot?
Best Regards
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