Hello everyone, fibroblasts recently arrived from ATCC. But in the laboratory we don‘t have the basal medium for fibroblasts. Can I do it with DMEM ?
Hello ! I seeded fibroblasts from ATCC PCS - 201 - 012. But they look round. They don't have an elongated shape And it's been 7 days. I used medium DMEM with 10% FBS and antibiotics, Fibroblast...
20 February 2024 8,023 4 View
If I convert J/m2 to W/m2 the reanalysis variable surface latent heat flux, will there be any difference with the variable mean surface latent heat flux? Or is it basically the same when you do...
15 September 2023 8,999 0 View
Steam es un movimiento innovador, sin embargo, me parece que carece de algunos elementos para considerarse modelo educativo.
08 July 2023 4,823 1 View
La idea es compartir experiencias del enfoque de la investigación de Gestión Visual, me pongo a disposición para platicar del tema, quedo atento a sus dudas o preguntas, saludos
06 March 2023 6,477 1 View
In the lab we use cotton seeds for DNA extraction. We extract DNA by adding a reagent and agitating, then we transfer a little to a TE plate and incubate at 60°C for one hour. I want to know why...
05 November 2019 9,671 4 View
I'm working in the validation of a 15 question long, Likert scale based questionnaire. The general steps I have followed are more or less described here:...
03 June 2018 6,425 5 View
I want to culture adult mouse cerebellum, but all protocols use neonatal or embrionic cells. Another question is if it´s reasoneable incubate whole cerebellum instead of slices?
06 December 2016 2,943 2 View
In the last decade, the population of butterflies has diminished in the colonies of hibernation in Mexico, so I want to know which are the impacts of the deforestation and what other consequences...
01 December 2016 7,291 4 View
I am dialysing a 40KDa protein in a 3Kda dialysis membrane. The buffer that my protein is in has some glycerol remaining. So I want to make sure the glycerol is not there anymore. I ran some...
27 April 2016 571 3 View
I am very interested to know whether the heritability of any behavior is greater than or equal to 50%, I would like to get more information on published "Behavioral profiles of dog breeds"...
26 October 2015 6,739 4 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...
01 August 2024 8,368 2 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Accredited laboratory of JV "Uz-Kor Gas Chemical" LLC (Uzbekistan) is looking for providers to conduct interlaboratory comparison tests on gas chromatographic analysis of hydrocarbons (component...
25 July 2024 8,108 0 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...
16 July 2024 6,918 7 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View