In the lab we use cotton seeds for DNA extraction. We extract DNA by adding a reagent and agitating, then we transfer a little to a TE plate and incubate at 60°C for one hour. I want to know why its relevant to incubate the DNA in TE.
TE contains EDTA which can inhibit some enzymatic reactions like ligation, blunting, and PCR, if it present in sufficient concentration. TE is a good choice to resuspend high-concentration stock DNA (like 100uM PCR primers) because you know it will "protect" your DNA long-term by buffering and chelation.
Dear Marisol
"TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation. EDTA chelates or binds Mg2+ present in the purified DNA and can help inhibit possible contaminating nuclease activity.
TE maintains the constant pH in solution. EDTA in TE buffer chelates the metal ions like Mg++ which act as cofactor in many enzymes like DNase, RNase etc. So chelating the cofactor, it deactivate those enzymes, thereby protect DNA.
TE provide conditional ph and edta chelate the ions required for enzymes responsible for cleavage of nucleic acid
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