Can I run solvent free reaction using biotage microwave initiator?

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Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to intepret data with significant ANOVA but not significant Tukey test?

I have this set of data: Treatment 1: 30.5; 34.5; 24.4 Treatment 2: 24.8; 20.8; 16.8 Treatment 3: 19.1; 21.4; 21.0 Treatment 4: 22.3; 26.1; 27.1...

25 November 2020 920 4 View

What can be the cause of nonspecific amplification in qPCR besides primer?

I adopt primer designs from a study published in Nature ( Domestication selected for deceleration of the circadian clo... ), so the primers should be qualified. Yet in many samples, I got...

31 August 2020 9,796 8 View

How to compare the difference between images from different modalities?

Hello all, At the moment, I am working on a project about the failure/fault detection on Photovoltaic Module based on Deep Learning using electroluminescence (EL) images. In this project, my...

23 August 2020 2,832 4 View

Frequentist vs. Bayesian methods for network meta-analysis?

Dear all, I want to conduct a network meta-analysis to find out the efficacy of different surgical interventions. I will use R as the main software. However, this is the first time I do a network...

21 June 2020 8,424 4 View

Can someone share with me the Dissolved Gas Analysis (DGA) dataset?

Hello scientists, We are proposing to develop a potential fault diagnostic model of Power Transformers according to the approach based on Hedge Algebra. Following this approach, we need to have...

03 April 2020 1,743 3 View

How to illustrate a data with time overlap and sizes?

I have data like this one: Study__ Periods_Control_Experiment Study1 1990-2000 100 50 Study2 1992-2005 78 90 Study3 1986-2009 98...

29 March 2020 7,330 3 View

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Did anyone transform un-digested pPICZalpha-A (circular DNA) which was inserted a desired gene into Pichia pastoris using electroporation? According to User Manual's Invitrogen (EasySelect Pichia...

01 March 2020 6,604 3 View

How to incoporate water insoluble drug into fast dissolving film to achieve a transparent film?

I've been preparing an oral dispersible film of a insoluble drug (Candesartan cilexetil). I used HPMC E5 as film forming, PEG400 as plasticizer. I tried using solid dispersion method taking...

19 February 2020 1,515 1 View

Does GC enhancer can be used in qPCR?

Hi everyone, I just want to know that it is any problems if i use GC enhancer or DMSO with my qPCR? Does anyones give me some advices? Thanks a lot.

17 September 2019 1,587 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View