You should treat your sample separately or tandemly for each N-linked and O-linked saccharide profile...You may follow endoglycosidase such as PNGase F for the enzymatic procedure at N-linked release. Beta alkaline elimination on the other hand is a chemical approach for O-linked...You may find many reagents and protocols on the Ludger website for glycan release. This would be the way for protein-bound carbohydrates. You can also unconjugate the bound carbohydrate variants by acid hydrolysis. But I am not sure this could reflect the whole spectrum.

One simple method for deglycosylation of proteins is using PNGase F, which is an enzyme that cleaves N-linked glycans from glycoproteins. Here is a step-by-step procedure for deglycosylation using PNGase F:

Note: The appropriate amount of PNGase F and reaction time may vary depending on the specific protein and experimental conditions, so it is important to optimize these parameters for each experiment.

This method is relatively simple and can be used for deglycosylation of a variety of glycoproteins, although it may not be suitable for all types of glycans. Other methods for deglycosylation include enzymatic or chemical methods, such as endoglycosidase H or peptide:N-glycosidase F (PNGase A), respectively. The choice of method depends on the specific glycan structure and the desired degree of deglycosylation.