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Questions related from Ravindra Deshpande
I am planning to stain a cell surface protein by immunofluorescence and then take readouts by plate reader. As I would like to detect cell surface protein, I guess cells should not be fixed. I...
08 August 2023 9,721 5 View
I was planning to evaluate the protein expression profile of a gene of my interest, in breast cancer patients. Does anyone know if such dataset ( like we use TCGA datasets to examine mRNA...
17 November 2022 7,630 2 View
I am having problem with real time (SYBR green) amplification of gene. I have designed primers spanning exon-intron junction. Primer con, was 1picomole. I have used 5micrograms of RNA for cDNA...
17 March 2015 904 11 View
Depending on their structure and molecular weight, how to check whether certain drugs or compounds crosses the blood brain barrier or not?
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I am planning to isolate some miRNAs fro human brain tissues as well as from cell lines. Can anyone please suggest kits?
30 April 2013 6,880 3 View
I am planning to detect expression of miRNAs in human tissues. Could anyone please let me know how to design primers/any online resources to design primers for miRNAs? Is it similar as we design...
27 April 2013 7,848 10 View
I would like to discuss various means to enhance the stability of synthesized C-DNA for further use as for PCR.
01 October 2012 345 3 View
