Ravindra Deshpande

6 Questions 10 Answers 0 Followers

Questions related from Ravindra Deshpande

Can I perform immunofluroscent staining in 96 well plate?

I am planning to stain a cell surface protein by immunofluorescence and then take readouts by plate reader. As I would like to detect cell surface protein, I guess cells should not be fixed. I...

08 August 2023 9,654 5 View

Proteomics dataset in breast cancer ?

I was planning to evaluate the protein expression profile of a gene of my interest, in breast cancer patients. Does anyone know if such dataset ( like we use TCGA datasets to examine mRNA...

17 November 2022 7,590 2 View

Any advice on my real time PCR amplification problem?

I am having problem with real time (SYBR green) amplification of gene. I have designed primers spanning exon-intron junction. Primer con, was 1picomole. I have used 5micrograms of RNA for cDNA...

17 March 2015 857 11 View

What are the best kits for miRNA isolation?

I am planning to isolate some miRNAs fro human brain tissues as well as from cell lines. Can anyone please suggest kits?

30 April 2013 6,841 3 View

Primer designing for miRNA.

I am planning to detect expression of miRNAs in human tissues. Could anyone please let me know how to design primers/any online resources to design primers for miRNAs? Is it similar as we design...

27 April 2013 7,821 10 View

How to enhance stability of c-DNA?

I would like to discuss various means to enhance the stability of synthesized C-DNA for further use as for PCR.

01 October 2012 300 3 View