I want to know if i can normalize the activity of an enzyme (eg Catalase ) by total protein (bradford) if my treatment affects the content of total protein content?
When i do the stadistical test i found SD in total protein content between tratments, so i can use this data to normalizethe activity of an enzyme?
An alternative would be to normalize to total mass of the sample (dry weight or wet weight) or to the number of cells, depending on what the sample is.
Hi Isis, catalase activity (V) per unit protein using the same assay can be defined as "specific activity"
Hi! First of all thanks a lot for your ansewrs,
The problem i found is that the number of cells also is affected by the tratments. So i could normalize, it is correct?
Hi Isis,
I think normalizing by cell number will give you an idea of whether the catalase activity of each cell was affected, independently of the effects on cell number.
Hi Isis. Usually, higher cell numbers = higher protein concentrations in your cell lysates.
If you really want to be thorough, you can isolate specific cell numbers and measure the total cell protein concentrations +/- treatment and plot it.
I would be surprised if there was a difference.
Hi
The number of cells is affected by the tratments, so why don't you check the significance of this phenomenon? I think this will be an interesting result, because if so, it means the treatment can cause cell lethality.
Also, i think the normalization by either cell number or total protein is convenient to analysis the activity of an enzyme.
Hi Isis,
I think normalizing by cell number will give you knowledge about the catalase activity of each cell was affected, independently of the effects on cell number.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology
- Enzymes