Can I make amplicons for sequencing using Real-Time PCR? Will probes interfere?

03 March 2013 0 9K Report

We want to sequence the pfcrt gene from Plasmodium malaria. But first, we have to perform amplification of that gene. I was wondering if using a stratagene thermal cycler (which uses strip with individual wells) for my pre-sequencing PCR would work?

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Science method

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