Hi,

I have transfected HeLa cells with pCDNA3 eGFP  by a calcium phosphate transfection protocol. I was wondering if I can maintain the fluorescence expression over time by passaging. Every week, I transfect a new batch of HeLa cells with the plasmid and this takes considerable time and labor on my side, so Im thinking if passaging these transfected cells would be okay to do to save some time and effort. I've never tried it so I'm happy to hear your opinions on how to go about this. 

Thank you very much! 

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