Michael McAlpine
Hello Vero
It is probably possible to leave your membrane overnight after transfer, but I'm not sure of how you would do this. I would personally recommend blocking the membrane immediately after transfer, as the pores of the membrane will be open to binding any protein after transfer increasing the background detected if the membrane is not blocked. In my lab we commonly block immediately after transfer, then probe the membrane overnight with our primary antibody at 4 degrees I would personally recommend this). But if you are low on time you should be able to block the membrane overnight at 4 degrees celsius and not observe any negative effects (However, you'll have to see if it works for your protein of interest)
Hope that helps
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