06 October 2022 1 9K Report

Instead of inserting my transformed plasmids (my sequences of interest) with the transposase mRNA in zebrafish embryo, can I just insert both plasmids at the same time?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to develop an academic literacy program for engineering at the higher education level?

Information literacy in higher education integration with curricula engineering

04 August 2024 5,368 3 View

Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

What should be the best Lumens range for T8 (120cm) full spectrum LED lamp tubes?

Please (for Arabidopsis), what could be a good Lumens and color range (Kelvin) range for full spectrum LED lamp tubes size T8 (120cm) for each shelve measuring 130x50 cm (length x width) and 60 cm...

11 July 2024 6,078 1 View

I need help with cloning a 200 bp insert and a 4,7 Kb pEGFP-N1 vector. How can I do it?

I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...

09 July 2024 4,277 1 View

Cross Attention in Transformers: Standard applications of the same ?

What are the standard applications of Cross Attention in Transformer Architectures ?

09 July 2024 9,310 2 View

Time Series Analysis: Has Recurrent Neural Networks (RNN) ever been used on Time Series Analysis ?

Are there standard RNN architectures been applied for Time Series Analysis, forecasting and anomaly detection problems ?

30 June 2024 3,169 8 View

LSTM on Time Series: Has LSTM architectures ever been applied to Time-Series Forecasting ?

Have we ever used LSTM architectures on Time-Series Forecasting and Analysis, and gotten a decent result ?

30 June 2024 6,924 3 View

What could be causing these smears in my PCR electrophoresis gel?

I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...

30 June 2024 4,107 4 View

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How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

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Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

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Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

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Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

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Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

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Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

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How Can I Apply Hashin Damage Properties to Solid Elements in Abaqus Without Using VUMAT?

Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...

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Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

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