I am conducting experiments on hormone secretion and hypothesize my compounds induced secretion in a calcium-independent manner. Can I incubate cells in HBSS without Ca and Mg for about 4 hours? Is this a good design?
I just tried this out and checked cell viability using trypan blue exclusion and MTT. Cells were fine during the test period.
To be on the safe side, I would eliminate only one of Ca and Mg for four hours.
Ok. Thanks. I think that's a better idea
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