Can I incubate cells in hanks balanced salt solution without Ca & Mg?

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Methods of teaching economics?

Answer should be based on institutional level

29 July 2024 1,506 4 View

How long can a whole blood sample stay after collection and still retain its integrity?

Can I use a whole blood sample maintain at a temperature between 4-8 degree Celsius for an enzyme assay after 24hrs?

05 April 2022 2,328 3 View

Good morning. I need articles and journals on emission control of Internal combustion engines ; modification of aqua silencer using nano tubes?

Nano tubes are polymers having manganese in it which will trap exhaust pollutants inside it and separate the hydrogen molecules. The hydrogen molecules can be used as regenerative to charge the...

11 March 2021 3,608 3 View

Interview questions analysis?

So i am seriously thinking about the use of interview on one of the studies I am presently conducting, however, I want to confirm if it is possible to analyze the response of respondents with the...

30 January 2020 10,018 7 View

Micro pension plan and informal sector workers?

Hello scholars, I've been wondering about this for sometime. How effective is micro pension plan in your country? The Nigerian government officially launched the plan in 2019. However, I seriously...

26 January 2020 5,325 2 View

Review of literature in journal articles?

Does review of literature in a journal article have to be comprehensive? Also, does it have to follow the standard format of conceptual Framework, theoretical Framework and empirical Framework?...

06 October 2019 972 12 View

Can I incubate cells in hanks balanced salt solution without Ca & Mg?

I am conducting experiments on hormone secretion and hypothesize my compounds induced secretion in a calcium-independent manner. Can I incubate cells in HBSS without Ca and Mg for about 4 hours?...

25 December 2017 8,708 4 View

How can i calculate the efficiency of dye-sensitized solar cell?

i have some problem with my I-V graph. can i calculate the efficiency only with open circuit voltage, short circuit current and light intensity?

04 May 2017 285 1 View

Increasing CT values on repeat PCRs of the same target and conditions?

I made an RT prep from cultured cells and am running PCR on the resulting cdna. Each time I run Bact on the same samples using the same primers and conditions I get a different result. Most...

05 October 2016 2,372 3 View

What exactly is the role of insulin in insulin resistance?

The role of hyperglycemia, saturated fatty acids, oxidative stress, inflammatory cytokines have been investigated in causing insulin resistance. However, is hyperinsulinemia itself not likely to...

17 September 2014 868 34 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

How can AI-driven personalized learning balance individual instruction with fostering social and collaborative skills?

This question tackles the complex issue of ensuring that while AI can tailor educational experiences to individual needs, it doesn't neglect the equally important aspect of fostering social...

02 August 2024 4,751 3 View

Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

What it is, that we do not know about the possible Social Impact of the AI on Society. Education, Human Computer Interaction and the Global Economy?

As with most changes in life, there will be positive and negative impacts on society as artificial intelligence continues to transform the world we live in. How that will balance out is anyone’s...

20 July 2024 10,062 3 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View