Can I incubate cells in hanks balanced salt solution without Ca & Mg?

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Alli-Oluwafuyi Abdul-Musawwir

I just tried this out and checked cell viability using trypan blue exclusion and MTT. Cells were fine during the test period.

Kirk MacGregor

To be on the safe side, I would eliminate only one of Ca and Mg for four hours.

Ok. Thanks. I think that's a better idea

Adam L Vanwert

Magnesium is essential for many metabolic functions in cells. It may be quite toxic to exclude it for more than 15 minutes.