If you're using just a basic visual dot probe paradigm; i.e., flanking a visual fixation cross with two images and then replacing an image with a dot, it's not a particularly difficult task to create this in Eprime. I have experience with using Eprime for other cognitive tasks, for example Posner's cueing paradigm (detection and discrimination), and that has worked quite well.
One consideration that you should take note of, though, is the stimulus display duration - Eprime isn't the greatest when it comes down to very quick display times (anything presented under 80-100ms I tend not to trust and would advise using Matlab to generate an experimental script). If you're using a standard image presentation of around 500ms and for the dot around 100-200ms you should be fine!
I also recommend having a look at the user guide for Eprime; it's very intuitive and will show you how to present this information.
We can implement a VDP task with our stimulus-presentation tool, EventIDE (www.okazolab.com), and then send you for testing. The task is a really simply and we guarantee accuracy at one monitor frame (~10ms), if needed. You can email me a description of your experiment ([email protected]) and I will contact you back.
Do you have experience with both E-Prime and Inquisit? I am trying to decide which one to use to implement the Dot Probe while the main criterion is the ease of use - which one is easier to master - to learn and use?