Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

Any recommendations for open-source survey form builder, which has option for semantic differential scale questions apart from Likert scale?

Asking this as Google forms doesn't allow multiple items in a semantic differential format.

04 February 2021 5,869 4 View

Can enzyme that is extracted from Aspergillus niger be used in the range of 4-40% v/v for optimization of veggie juices like broccoli??

09 December 2020 7,083 3 View

Genomic DNA Exteraction from Asp nidulans?

Hi All, I have several trials for few months to extract good genomic DNA from some cultures of Asp. nidulans, most of them worked with CTAB extraction from freez-dried mycelium (30-40-60 hrs old)...

21 October 2020 6,030 3 View

Calcofluor white staining works with Aspergillus niger spores?

I'm trying to obtain colonies with only mycelia and without spores. I know you can use the calcofluor white staining for the fungi because it binds the cell wall of spores and it's fluorescent....

01 August 2020 6,952 2 View

How many references sequences should use for a phylogenetic tree construction?

I have identified fungus isolates from the fruit surface by SSU +ITS+ LSU regions, such as : 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus...

15 July 2020 5,573 2 View

Do lower nanoparticle concentrations give better results than higher concentrations?

When using one of the nano particle to inhibit the growth of the fungus Aspergillus flavus on the culture medium PDA, the lower concentration gave better inhibition rates than the higher...

07 July 2020 7,722 11 View

How old is the "fungal isolate" most suitable for pathogenicity testing on the surface of fruits?

I have different fungus isolates such as; 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus ; 5) Aspergillus flavus ; 6) Penicillium...

01 July 2020 6,889 3 View

Is the genus Neosartorya one of the telomorphs of the Aspergillus genus? Are there other telomorphs of all species of Aspergillus?

While discovering the types of Aspergills, I found a colony in white color that has microscopic structures similar to Aspergillus microscopic structures. When I used the Aspergill keys he...

07 March 2020 1,605 3 View

How do I measure collagen deposition in asthmatic mouse lung using ImageJ?

Hello All I am working on allergic asthma and hyper-responsiveness in mouse exposed to Aspergillus fumigatus. I am trying to measure collagen deposition using the ImageJ software but i don't know...

02 March 2020 6,830 3 View

How to prepare inoculum for aspergillus sp. for BMD testing?

we do not have UV-Vis spectrophotometer to adjust OD at 530 nm which is desirable for inoculum preparation of non dermatophyte. Is there any alternative method such as Barium chloride turbidity...

21 February 2020 3,975 2 View

How to stain Aspergillus DNA with Hoechst 33258 ?

Hey, i want to stain my Tet-On Strain of A. niger with Hoechst 33258 for fluorescence microscopy. Do you have any advice for me ? Best wishes David

31 December 2019 7,901 2 View