Hi everybody,

I'm planning some in vivo work with my liposomal drug delivery devices and want to do SPECT imaging for biodistribution and pharmacokinetic characterization of my liposomes and would like some input on methods for radiolabeling of liposomes.

I'm using 111-In as a radionuclide, and I'm wondering what method for radiolabeling would be the best?

Either using functionalized PEG-chains with either amine or maleimide and doing a "post-insertion" method similar to the one used for antibody grafting or remote loading the radionuclide, along with a chelator, in the liposomal aqueous compartment?