Hi guys,
I'm planning to do Blue Native PAGE.
Are there any problems when i use buffer composition bellow ?
Electrophoresis Buffer (1L)
3.0g Tris
14.4g Glycine
5x Sample Buffer (10ml)
312.5 mM Tris-HCL(pH6.8)
50% Glycerol
1% Comassie blue
Hi Jinsoo Kim,
Please read the following articles.
Article Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for...
https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf
Article Blue Native Polyacrylamide Gel Electrophoresis (BN‐PAGE) for...
Article Chapter 13 Blue-native gels to isolate protein complexes fro...
Regards
Unless you are able to calculate a DISC buffer composition (see Orntein's paper), you will be better off using the published systems. Any deviation will cause a loss of resolution.
The composition suggested looks ok. Give a try with your sample. The electrophoresis buffer must be alkaline with a pH 8.6.
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