that's the question.
thank you!
The effect of the special program in the arts (SPA) curriculum to the completers.
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Dear connections, I am currently involved in a project examining the BODY LANGUAGE OF ELITE COACHES and its potential influence on athletes. However, there seem to be a lack of instruments...
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I am an undergraduate and I would like to broaden my knowledge on this subject, beginning by its principles. Maybe it would help that purpose to broaden my knowledge on Phylogenetics as well....
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Dear colleagues, I would like to know your considerations about these descriptors, related to our knowledge field. Thank you in advance.
12 December 2019 1,755 20 View
There is a paradigm shift of the UPP, which leads to call them ulcers related to dependence. In the critical patient in intensive care unit, we monitor parameters that can help to understand the...
12 February 2019 4,869 0 View
Hi, I'm preparing a PhD proposal to study an egocentric network in primary health care setting in a low-middle income country feed with a name-generator survey. I have 2 questions: 1. Any...
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The boy has received treatment with steroids, cyclophosphamide and cyclosporine A, without a good response.
27 September 2016 5,049 4 View
Dear all, I am conducting a study on community composition and stable isotope signatures of arthropods. I use PERMANOVA+ for PRIMER v.6. In several tests I use two random factors. One related to...
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With the purpose of acquiring information for robotic systems, the image of a configuration space must be filled with grids. This information is useful for robotic systems because it provides a...
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
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How to design VN primer to attach with universal reverse primer
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Would anyone be able to advise me... I have an RCT with a control and experimental group. Participants were recruited from one school (n=59). Participants were assessed using repeated measures on...
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It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
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Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
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Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
28 July 2024 7,827 6 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
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