How much Morphine Standard is dire need for determination and extraction of Morphine in HPLC technique?

I want to purchase some Morphine Standard for HPLC to detect Morphine rate in Papaver somniferum L. how much is need in every stage or phase?

08 January 2021 1,675 3 View

Hi dear fellow researchers and senior scientists ,i need validate method for Safranin-O-fast green images intensity quantification?

Dear fellows i have images of meniscus sections stained with Safranin-O-fast green. I need to quantify the images for Safranin-O-fast green intensity and any other parameter if...

30 December 2020 1,931 3 View

What is the blank for catalase activity measurement by spectrophotometer?

I extracted the enzymes of leaves with a potassium phosphate buffer containing PVP and EDTA for catalase activity measurement. The reaction mixture is 3 ml (2.95 ml phosphate buffer+ 0.05 ml...

26 December 2020 606 3 View

How to model linear-elastic large deformation of 1D Uniaxial stress in ABAQUS?

Dear researchers, how to model linear-elastic large deformation of 1D Uniaxial stress in ABAQUS? How does the abaqus distinguish between the 1d uniaxial stress and strain analysis? Thanks in advance.

20 December 2020 370 2 View

Can you accept for my invitation to join the JAC-ECC Conference, I enclose for you the detailed program?

can you accept for my invitation to join the JAC-ECC Conference, I enclose for you the detailed program?

07 December 2020 8,576 7 View

How I can create neural network model for simultaneous determination of mixture of two drugs?

I want to help me in creating neural network for simultaneous determination of two drugs. I make 26 mixture of the two drugs this is my inputs data, the spectrum of the mixture of the two drugs...

19 May 2020 10,005 3 View

How I can quantify H&E histology image for tissue using Fiji?

H&E histology quantitative analysis using software

17 May 2020 2,233 10 View

I want to use principle component analysis using matlab to simultaneous spectrophotometric determination of two drugs ?

The attached file is matlab file contains matrix (A) for the absorbance of the two compounds, the first 9 column is for compound x and the last 10 column is for compound y matrix C for...

13 April 2020 5,031 2 View

I am looking to hear someone recommend me A simulating software which can be use to Analyze FYP project ""Peer to Peer or P2P Solar Energy trading "?

I am really confused now which simulation i can use to analyze.

07 February 2020 4,325 3 View

How can we prepare meta-regression model for different clinical trails? Which software is free for this?

how can we prepare the meta-regression model for different clinical trails? Which software is free for this

31 January 2020 2,402 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View