I am looking for a details protocol of attaching suspension cells to slides for staining.
Thanks in advance.
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I am able to prepare Go and Rgo which I have confirmed with Xrd data but sponge formation is difficult. initially solid sponge type structure was formed but it was Fragile . Actually my main...
22 May 2024 1,056 0 View
Our Universe has Visible and Dark matters that we believe but we can see between planets or heavenly bodies that have much distance, They have their magnetic field too. Whether they are situated...
05 March 2024 8,944 5 View
What is the best International trade textbook for undergraduate Level students?
09 February 2024 2,153 8 View
I'm facing a puzzling question. I stained two different cell lines of the same type and observed DAPI dots in the cytoplasm of one, unlike the typical DAPI pattern in the other (see images). Both...
17 January 2024 3,300 5 View
Giving advice is a matter of honesty and we must advice in the best interest of the companion. Hope getting good views and suggestion on this topic. Thanks & Regards
10 January 2024 5,503 1 View
the answer is needed for super capacitor investigations and development
21 December 2023 1,500 3 View
We are optimizing SDS-PAGE, i would like to know the best ratio and percentage for the Staking gel and separation gel of Acrylamide. Its like 37.1:1 and 29.1 and 19.1 and also 30% and 40%, So i...
06 December 2023 1,080 6 View
Dear Researcher Which course title is good for PhD level Students in Monetary Economics Advanced Monetary Economics Advanced Monetary Theory Monetary Thoery and policy Applied Monetary...
25 November 2023 4,576 3 View
While I simulating my optical fiber design, I get some positive and some negative values of refractive index imaginary part. As I know, I must get negative values to compute confinement loss. I...
10 November 2023 9,219 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View