I'm facing a puzzling question. I stained two different cell lines of the same type and observed DAPI dots in the cytoplasm of one, unlike the typical DAPI pattern in the other (see images). Both were untreated and on the same plate, subjected to similar treatment.
Has anyone encountered a similar issue? Any hypothesis of what can it be? We have done mutiple mycoplasma testing by PCR and turned out negative. Also, if contamination is present and the cells share the same plate, shouldn't the contamination transfer between them?
thank you very much for your help
This is probably due to chromosomal instability where whole chromosomes or fragments of chromosomes are mis-segregated during mitosis. The cell line giving that is a cancer cell line? That would explain it.
Hi thank you Christopher and Anna for your replies. These are ipsc derived cardiomyocytes so they are supposedly undividing cells. We still haven’t figure out what it can be. We still see the same profile in this line and we are seing it in another line now. Meanwhile the pcr test are still negative.
Mitochondria? Cardiomyocytes have much more mitochondria compared to the other cells...
Christopher B O'Connell is correct and these structures are called micronuclei, see for example https://en.wikipedia.org/wiki/Micronucleus
thank you for your answer. These cells don’t proliferate. We have stained them multiple time and never seen the dots in DAPI. we started to see the same profile in another line. we are really doubting mycoplasma but all the tests are negative so far
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