Can I freeze E.coli stock at -20C?

Lisa Corcoran @Lisa-Corcoran-6

29 June 2022 0 8K Report

Can I make a 20% glycerol stock and freeze it at -20C? We don't have access to a -80C freezer.

Badges
Science topic

More Lisa Corcoran's questions See All

Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Articles on" Gender disparities i leatherwork education"?

Articles on" Gender disparities i leatherwork education"

07 August 2024 2,500 0 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Shortage Cost Hypothesis?

What can be a hypothesis related to the Stock out Cost or Shortage Cost?

29 July 2024 9,259 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

Suggestions for storage of CD4+ naive splenocytes for future use?

Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...

02 July 2024 4,887 0 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View