Can I develop mice glioblastoma model with glioblastoma cell line? If the answer is yes, what are the most important parts?

“Essentially, all models are wrong, but some are useful”

-George Edward Pelham Box-

So, you don’t have an infinite amount of resources, and life is short, so you don’t want to spend the rest of your degree optimizing your model. Hence, you must decide how ‘realistic’ you want your model to be, as well as decide what aspects of cancer biology your model need to recapitulate (or at least ‘mimic’ with some accuracy).

So, the answer to your question depend on this: what is your study question?

As an example, are you interested in testing a drug’s ability to kill cancer? Are you interested in the effect of cancer on the host (e.g. the development of cancer cachexia)? Are you aiming to elucidate the molecular events leading to metastasis of cancer cells? Interested in organ tropism of cancer cells (e.g. why do cancer cell so seldom metastasise to mussel, despite muscle tissue being well vascularised and representing a large fraction of our body weight)?

Here is an example:

If you are interested in developing/testing a cancer vaccine, it may be necessary to use a syngeneic mouse model (i.e. inject cancer cells derived from the same strain of mice as the mice you are injecting). To explain: If you want to inject human cancer cell into mice, you need an immune compromised mice, otherwise your mice will reject the tumour graft. But your mice need an intact adaptive immune system for the vaccine strategy to be realistic. (See how the study question ‘force’ the design your model system?)

There are numerous parameters that are important or irrelevant to your model, pending on the exact question you have (and the time and money you can spend on it). Thus, it is impossible to give you a clear-cut answer since we don’t know what your study question is (i.e. what your model need to do- in what way is it ‘okay’ for your model to be ‘wrong’ but still be ‘useful’?)

So, the ‘the most important parts’ are dependent on what question you what to answer in your study.

Robert Kiss

You very welcome Meryem!

Do not hesitate to come back with more precise questions if you need.

As Gustav said, a model remains a model.

But there are many different models with respect to GBM orthotopic syngeneic or xenograft models.

Depending on your working hypothesis, some models are more appropriate than others.

I can help if needed.

Best regards

Robert

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