I want to study glioblastoma in vitro and in vivo but i am not sure how can I develop mice glioblastoma model with glioblastoma cell line or can I ? And what are the most important points? If there are any suggestions, I will be thankful. With regards
“Essentially, all models are wrong, but some are useful”
-George Edward Pelham Box-
So, you don’t have an infinite amount of resources, and life is short, so you don’t want to spend the rest of your degree optimizing your model. Hence, you must decide how ‘realistic’ you want your model to be, as well as decide what aspects of cancer biology your model need to recapitulate (or at least ‘mimic’ with some accuracy).
So, the answer to your question depend on this: what is your study question?
As an example, are you interested in testing a drug’s ability to kill cancer? Are you interested in the effect of cancer on the host (e.g. the development of cancer cachexia)? Are you aiming to elucidate the molecular events leading to metastasis of cancer cells? Interested in organ tropism of cancer cells (e.g. why do cancer cell so seldom metastasise to mussel, despite muscle tissue being well vascularised and representing a large fraction of our body weight)?
Here is an example:
If you are interested in developing/testing a cancer vaccine, it may be necessary to use a syngeneic mouse model (i.e. inject cancer cells derived from the same strain of mice as the mice you are injecting). To explain: If you want to inject human cancer cell into mice, you need an immune compromised mice, otherwise your mice will reject the tumour graft. But your mice need an intact adaptive immune system for the vaccine strategy to be realistic. (See how the study question ‘force’ the design your model system?)
There are numerous parameters that are important or irrelevant to your model, pending on the exact question you have (and the time and money you can spend on it). Thus, it is impossible to give you a clear-cut answer since we don’t know what your study question is (i.e. what your model need to do- in what way is it ‘okay’ for your model to be ‘wrong’ but still be ‘useful’?)
So, the ‘the most important parts’ are dependent on what question you what to answer in your study.
Some of the attached articles can be helpful.
Using GBM cell lines in vitro or orthotopic xenografts in vivo are routine procedures.
Best regards
Robert
Thank you Robert and Gustav for your helpful answers. Your ideas and suggestions are really significant to me.
With regards
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line