Is valid this complexity theory technique to solve outstanding problems?

04 June 2020 7,203 2 View

Hello Who can help me with this error of hec hms ERROR 41742: Invalid initial loss for subbasin??

This error apears only in subasins with a value superior than 19, 682 in the initial abstraction in the method SCS Number Curve.

18 May 2020 6,985 3 View

Are they valid these complexity theory techniques to solve outstanding problem?

03 March 2020 1,035 5 View

Looking for very stable and innert dyes, any suggestion?

Hi. I am looking for dyes (preferably green or blue) that are very stable (time and temperature), do not change color between pH 6-9 and do NOT interact with proteins or sugars. Thanks

22 January 2020 4,593 5 View

Please, how to run a Cross-lagged Panel Model in Stata?

I am using panel data to search for the causality between two variables, and I think a Cross-lagged Panel Model would be appropriate. I have 8 waves and I want to run de model wave by wave in...

10 November 2019 9,736 4 View

How to growth spirulina in 1m3 tank?

I want to try different methodologies to grow spirulina in a 1 cubic meter tank and compare the production yield. Please if you have a tip, let me know.

13 September 2019 1,583 3 View

Anybody knows a way to identify antibodies in human plasma/ serum (for a rare disease)?

We have a research problem. We have various patients with a very unknown rare multisystem disease, of presumed autoimmune origin (since it can be managed with immunosuppressive agents.) We would...

03 April 2018 1,999 3 View

I would like to have a comparison between values and principles in nanotechnology... what do you think about it ?

I know that principles are universal values... is it true in nanotechnology ?. Scientist in nanotechnology think about it?

01 March 2018 2,706 4 View

What is the right way to check a mathematical model?

13 September 2017 5,210 5 View

What is the right way to check a mathematical model?

08 September 2017 9,225 6 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Having trouble mounting the sections on the slides for IHC staining, what solution should I use?

Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...

08 February 2021 6,880 3 View

How to fix and protect blood clots for histology ?

Hi, I encounter some problems, I need to my research to fix and cryoprotec some blood clot for histology analyses. Mys current protocol is the following : 48h of para formaldehyde 4% and 7 days of...

07 February 2021 3,756 3 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Which isomer of xylene do you use in hystology ?

Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?

02 February 2021 833 4 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View