My RNA concentrations from brain tissue were at highest around 80ng/mL and as low as 5ng/mL. Can I converse my RNA to cDNA with this low concentration of RNA?
I'm analyzing the sub-surface stresses in a line contact in combination with a tangential load (sliding cylinder). I can calculate Sxx and Szz (Z is the normal to the contact surface). And Syy is...
23 November 2020 894 0 View
I have a question about protein transfection. I used a reagent to transfect a protein. Can I use the same reagent for a different protein that I want to transfect in the same cell line?
31 May 2020 2,750 3 View
Hello, In qPCR, is there an optimal concentration for primers with a total volume of 20ul per reaction? Thanks for your answer in advance!
24 May 2020 2,092 9 View
Can somebody help me with the following question: Is there a certain duration for gene knockdown using shRNA or is the knockdown permanent? Thanks and stay save!
05 April 2020 7,069 2 View
Good morning, Right now, I’m trying to express thermophilic genes in E.coli. On the internet I have read that I need to incubate the cells at 42 degrees for a short time period after IPTG...
08 March 2020 7,747 1 View
I want to perform DNA methylation profiling in leukocytes for an epigenetic analysis. However, I want to preserve the leukocytes for DNA methylation profiling for later. What is the best way to...
01 March 2020 7,691 3 View
I want to synthesize cDNA for qPCR using the iScript cDNA synthesis kit. I suspended the RNA in TE buffer with 1mM EDTA. Does the EDTA have an influence on the synthesis of the cDNA. Note: the...
23 February 2020 7,798 3 View
Fixed seat rowing introduces pitching and heaving motions, especially at the start of the recovery, as well as a surge. How does the magnitude of the thus added resistance compare to the linear...
10 April 2016 5,952 1 View
What was the main variable? Backbone or blend composition, or other? I am looking for a grade or blend or compound of polypropylene with the lowest possible shrinkage upon cooling after thermal...
28 October 2015 4,874 4 View
Inertial MEMS give a small capacitive signal and are sensitive to vibrations. In a multi-chip module (MCM) wire bonding is applied to connect with a sense and control circuit (ASIC). What are the...
03 June 2014 8,892 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View