TC-2 Tissue sectioner SORVALL?

I bought this tissue sectioner SORVALL TC-2, but I do not find the manual and I would like to know if someone uses this machine and can help me to understand how it works.

01 December 2020 550 2 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

Carbon content over 100 in a charcoal sample under treatment at 1200 C?

After elemental analysis of the samples, carbon content of the charcoal was 102 (several repetitions were made, but obtained the same values), the equipment was tested with other samples of wood...

02 September 2020 1,971 2 View

Can anyone help me with my contaminated human muscle cells in culture?

I am facing a problem with cell culture for weeks now. The media of the cell culture turnes turbid (white little spots, see picture) but stays reddish/pink. First this happened after 2 weeks of...

01 September 2020 1,498 9 View

Chi-Square Goodness of Fit: Can I hypothesize an expected outcome of 0 for one variable and 100 for the other variable?

If you have three variables A, B, C and you want to test whether the hypothesis is that everyone focuses on C, can the expected outcome in a chi-square goodness of fit test then be A: 0 B:0 and C:...

28 May 2020 2,008 6 View

Does anybody have a good Cytoplasmic Membrane stainning protocol?

11 February 2020 6,922 3 View

Which antibody for mouse p300 IP?

Which is the best Antibody to perform IP of endogenous mouse p300 IP. Please provide a figure or a citation in your answer. Thank you!

24 September 2019 8,482 2 View

How can I compute a factor (latent variable) knowing the variables that contribute?

Dear all, I have a data set with 7 variables. I have run a EFA and I got three factors. I want to obtain a numerical value for each factor computed from the variables that form it but I am not...

20 January 2019 1,933 3 View

Any body working on spectroscopic investigations on functionalised fullerenes. experimental and theoretical approach

want to contact the person working in the field

08 July 2017 1,370 2 View

Negative Results on Nanodrop

I am having negative results for my DNA/RNA extraction from stool samples on Nanodrop for Concentration, 260/280 and 260/230. This is the first time I'm having this problem and have tried to use...

17 June 2017 4,774 12 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

How to correctly choose the number of solution intervals when preforming harmonic analysis in ANSYS?

Good day, I design the rail-wheel geometry and the harmonic frequency ranges between 20-10kHz with 500Hz solution intervals and the results makes sense. Most of the time on the related literatures...

19 February 2021 3,190 3 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

Can anyone name this graph type?

I've attached a recreation of this graph type that I need to make for study reports. The clients have sent them to us in the past, but I am trying to make the reporting process more streamlined,...

15 February 2021 9,795 3 View