I have seen papers that derive LOD and sensitivity from constant potential amperometry data (CPA or step current experiment), the raw data for which looks something like the attached figure. A calibration plot is derived from the peak currents and LOD and sensitivity calculations are done.
I'm wondering if the LOD and sensitivity calculations can be done using the calibration plot derived from the peak currents obtained from cyclic voltammetry (CV)? However, in CV the solution is unstirred as opposed to the CPA study where the solution is constantly stirring through the experiment.
I understand that response time calculated from an unstirred CV study may not be of significance but what about LOD and sensitivity?
The detection limit, lower limit of detection, or LOD (limit of detection), is the lowest quantity of a substance that can be distinguished from the absence of that substance (a blank value) within a stated confidence. the relationship between the blank, the limit of detection (LOD), The LOD defined as 3 * standard deviation of the blank for a Method detection limit.
Sensitivity is the magnitude of the response per unit concentration. It can be calculated from the slope of the calibartion curve from linear range/ working area of the electrode.
Please use standard deviation method from slope of linearity curves. I.e LOD = (3.3xSD)/Slope
You can see this work to calculate properly the LOD. http://dx.doi.org/10.1016/j.snb.2012.06.071
Respecting to CV data, you can make your calibration curve with unstirred solutions by graphing ip data vs concentration. But your voltammograms must be corrected at the baseline in order to read properly the ip.
http://dx.doi.org/10.1016/j.snb.2012.06.071
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