Can I alkylate (e.g. iodoacetamide) solvent exposed cysteines of a purified membrane protein??

I have purified a transporter membrane protein and want to perform a CPM assay to assess its melting temperature as apo- and with ligands. The melt scan results in steadily dropping curve while my...

26 September 2020 1,455 4 View

How do I compare various cox proportional hazard regression models in SPSS?

I have generated various models with an identical core of fixed covariates with each model differing only in the study covariate (a categorical variable that I change in each model by altering...

14 September 2019 2,397 2 View

Why in 211 MAX phase publications mainly Ti2AlC, Ti2AlN and Cr2AlC are considered?

There is a plethora of possible MAX phases, but from what i have seen, papers tend to focus mainly on those three. What about all the other ones? Are they experimentally not feasible?

12 March 2019 4,300 6 View

What does it mean if I find more than one STR in a sequence?

I used TandemRepeatFinder to find STRs in sequences I retrieved from Genbank and in some sequences the program has found more than one STR. What does this mean? In a sequence for example, some of...

30 March 2018 3,794 1 View

What is your opinion about Fritz Haber?

02 April 2017 6,948 1 View

How to aplpy periodic boundary conditions on a cubic rve of a cellular solid using ANSYS/APDL(any code,advice,example would be very useful)?

Any type of help (guidelines,advice codes,examples) would be much appreciated since i'm having a tough time applying PBC in Ansys(APDL).

04 July 2016 6,441 2 View

Can we use Busch theorem to find the beam radius and the Larmor radius at any axial position?

The question is fairly simple In the book "Gyrotrons: High-Power Microwave and Millimeter Wave Technology" from M. V. Kartikeyan, Edith Borie, Manfred Thumm, in page 107 a generalization of Busch...

21 May 2015 1,281 2 View

Is there a resource for the current Tree of Life, based on published research?

I need to access global phylogenies, going down to the species level if possible. Is there a resource that provides different resolutions of the Tree of Life, based on our current knowledge about it?

01 February 2014 6,647 7 View

Any suggestions for synchronizing TM4 Sertoli cells?

I've recently thawed a TM4 Sertoli cell line, which I'd like to stimulate with a specific substance, but before I do that I want to synchronize it by using a specific Hunger medium. I've read...

28 January 2014 1,877 3 View

If we were to consider a concept to be the irreducible quantum of consciousness, how can this be applied and used in Cognitive Neuroscience?

A concept is not a mere group of coactivating synapses or a pattern of neuronal activity. It is the recognizable awareness of this underlying event. I came to the realization that a concept may...

30 August 2013 8,109 34 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View