Can I apply Taqman qRT-PCR primer set to SYBR Green qRT-PCR?

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Babak Nami

sure, but consider 2 important matters. first, your primers must does not form self- and hetero-dimer, and second, the amplicon length should be in range 60-120 bps for applying in Sybr Green dye qPCR.

Good luck

@Steve: Hi, thats true for all PCR but the rules is not critical for Taqman system than SYBR Green

Kang Mo Ku

Good Thank you for your valuable opinions!!